Thymic stromal lymphopoietin is regulated by the intracellular calcium

被引：20

作者：

Han, Na-Ra ^{[1
]}

Kim, Hyung-Min ^{[1
]}

Jeong, Hyun-Ja ^{[2
]}

机构：

[1] Kyung Hee Univ, Coll Oriental Med, Dept Pharmacol, Seoul 130701, South Korea

[2] Hoseo Univ, Biochip Res Ctr, Asan 336795, Chungnam, South Korea

来源：

CYTOKINE | 2012年 / 59卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

Calcium; Caspase-1; Receptor interacting protein2; Thymic stromal lymphopoietin; NF-KAPPA-B; SMOOTH-MUSCLE-CELLS; MAST-CELLS; TNF-ALPHA; PATHWAY; EXPRESSION; KINASE; TSLP; IL-6; CA2+;

D O I：

10.1016/j.cyto.2012.04.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The cytokine thymic stromal lymphopoietin (TSLP) has been implicated in the development and progression of allergic diseases such as atopic dermatitis. However, it has not been clarified that TSLP would be regulated by intracellular calcium in mast cells yet. To determine it, we blocked intracellular calcium by treatment with calcium chelator, 2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester (BAPTA-AM) in human mast cell line (HMC-1) cells. BAPTA-AM inhibited the production and mRNA expression of TSLP in phorbol myristate acetate plus A23187- stimulated HMC-1 cells. BAPTA-AM also inhibited the nuclear factor-kappa B activation, I kappa B alpha phosphorylation, receptor interacting protein2 (RIP2) expression, and caspase-1 activation in HMC-1 cells. These results provide evidence that calcium regulates the level of TSLP through RIP2/caspase-1/NF-kappa B/I kappa B alpha signal. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：215 / 217

页数：3

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