Separation of EPA and DHA in fish oil by lipase-catalyzed esterification with glycerol

被引:36
作者
Halldorsson, A [1 ]
Kristinsson, B [1 ]
Glynn, C [1 ]
Haraldsson, GG [1 ]
机构
[1] Univ Iceland, Inst Sci, IS-107 Reykjavik, Iceland
关键词
DHA; EPA; esterification; n-3 fatty acids; fish oil; glycerol; kinetic resolution; lipase; Lipozyme RM IM; Rhizomucor miehei lipase;
D O I
10.1007/s11746-003-0796-8
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The objective of this study was to investigate the use of lipases as catalysts for separating EPA and DHA in fish oil by kinetic resolution based on their FA selectivity. Esterification of FFA from various types of fish oils with glycerol by immobilized Rhizomucor miehei lipase under water-deficient, solvent-free conditions resulted in a highly efficient separation of EPA and DHA. Reactions were conducted at 40degreesC with a 10% dosage of the lipase preparation under vacuum to remove the coproduced water, thus rapidly shifting the reaction toward the products. The bulk of the FA, together with EPA, were converted into acylglycerols, whereas DHA remained in the residual FFA. As an example, when FFA from tuna oil comprising 5% EPA and 25% DHA were esterified with glycerol, 90% conversion into acylglycerols was obtained after 48 h. The residual FFA contained 78% DHA and only 3% EPA, in 79% DHA recovery. EPA recovery in the acylglycerol fraction was 91%. The type of fish oil and extent of conversion were highly important parameters in controlling the degree of concentration.
引用
收藏
页码:915 / 921
页数:7
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