Temporal accumulation of salicylic acid activates the defense response against Colletotrichum in strawberry

被引:26
作者
Grellet-Bournonville, Carlos F. [1 ,2 ]
Martinez-Zamora, Martin G. [1 ,2 ]
Castagnaro, Atilio P. [1 ,2 ,3 ]
Carlos Diaz-Ricci, Juan [1 ,2 ]
机构
[1] Univ Nacl Tucuman, Inst Super Invest Biol CONICET UNT, Dept Bioquim Nutr, RA-4000 San Miguel De Tucuman, Tucuman, Argentina
[2] Univ Nacl Tucuman, Inst Quim Biol Dr Bernabe Bloj, RA-4000 San Miguel De Tucuman, Tucuman, Argentina
[3] Unidad Asociada INSIBIO, Secc Biotecnol, Estn Expt Agroind Obispo Colombres EEAOC, RA-4101 Las Talitas, Tucuman, Argentina
关键词
Strawberry; Defense response; Salicylic acid; Anthracnose; Colletotrichum; SYSTEMIC ACQUIRED-RESISTANCE; PATHOGENESIS-RELATED PROTEINS; TRANSLOCATED SIGNAL; POWDERY MILDEW; TOBACCO; EXPRESSION; INDUCTION; VIRUS; ARABIDOPSIS; BENZOTHIADIAZOLE;
D O I
10.1016/j.plaphy.2012.01.019
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Many authors have reported interactions between strawberry cultivars and pathogenic microorganisms, yet little is known about the mechanisms triggered in the plant. In this paper we examine the participation of the salicylic acid (SA) signaling pathway involved in the response of Fragaria x ananassa cv. Pajaro plants to pathogens. Strawberry plants were challenged with the virulent strain M11 of Colletotrichum acutatum, or with the avirulent strain M23 of Colletotrichum fragariae which confers resistance to the former. Our study showed that the isolate M23 induced a temporal SA accumulation that was accompanied with the induction of PR-1 gene expression in strawberry plants. Such events occured after the oxidative burst, evaluated as the accumulation of hydrogen peroxide and superoxide anion, and many hours before the protection could be detected. Similar results were obtained with exogenously applied SA. Results obtained supports the hypothesis that strawberry plants activate a SA mediated defense mechanisms that is effective against a causal agent of anthracnose. In contrast, plants inoculated with M11 did not show oxidative burst, SA accumulation or PR1 gene induction. This is the first report about a defense response signaling pathway studied in strawberry plants. (C) 2012 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:10 / 16
页数:7
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