Development of microsatellite markers for Japanese scallop (Mizuhopecten yessoensis) and their application to a population genetic study

被引:74
作者
Sato, M
Kawamata, K
Zaslavskaya, N
Nakamura, A
Ohta, T
Nishikiori, T
Brykov, V
Nagashima, K
机构
[1] Hokkaido Food Proc Res Ctr, Ebetsu, Hokkaido 0690636, Japan
[2] Hokkaido Scallop Fishery Promot Cooperat, Chuo Ku, Sapporo, Hokkaido 0600003, Japan
[3] Hokkaido Wakkanai Fisheries Expt Stn, Wakkanai, Hokkaido 0970001, Japan
[4] Russian Acad Sci, Inst Marine Biol, Far E Div, Vladivostok 690041, Russia
关键词
scallop; population genetic structure; microsatellite marker; mitochondrial marker; polymorphism;
D O I
10.1007/s10126-004-0127-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The Japanese scallop (Mizuhopecten yessoensis) is one of the main fishery products in Japan, but with the expansion of culture operations of the Japanese scallop, various problems have been encountered including high mortality, poor growth, poor seed production, and so on. Moreover, there is concern that many years of cultivation may have affected the genetic structure of the scallop population. To approach these problems and concerns, we developed microsatellite markers as a molecular tool for population genetic studies. By using 4 microsatellite markers as well as a mitochondrial marker, we investigated the genetic structure of samples from the islands of Hokkaido (14 populations) and Honshu (Tohoku, 3 populations) in Japan, and south Primorye (4 populations) in Russia. All the populations sampled had high genetic diversity (average expected heterozygosity, 0.7011 to 0.7622; haplotype diversity, 0.6090 to 0.8848), and almost all showed a tendency of homozygote excess, which was significant in 2 populations. Hierarchical analysis of molecular variance tests based on the microsatellite and mitochondrial markers indicated that the 3 geographic regions were genetically divergent from one another, with little evidence of divergence within regions. Homogeneity in allele frequency distributions between natural and cultured scallops and allele frequency stability over a period of 2 decades indicated that the culturing operations have probably not had a substantial effect on the genetic structure of the populations.
引用
收藏
页码:713 / 728
页数:16
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