Pulmonary fibroblasts from COPD patients show an impaired response of elastin synthesis to TGF-β1

被引：15

作者：

Zhang, Jing ^{[2
]}

Wu, Lian ^{[1
]}

Feng, Ming-xiang ^{[3
]}

Sexton, Paul ^{[1
]}

Bai, Chun-xue ^{[2
]}

Qu, Jie-ming ^{[4
]}

Merrilees, Mervyn ^{[5
]}

Black, Peter N. ^{[1
]}

机构：

[1] Univ Auckland, Dept Pharmacol & Clin Pharmacol, Auckland 1, New Zealand

[2] Fudan Univ, Shanghai Med Coll, Zhongshan Hosp, Dept Pulm Med, Shanghai 200433, Peoples R China

[3] Fudan Univ, Shanghai Med Coll, Zhongshan Hosp, Dept Thorac Surg, Shanghai 200433, Peoples R China

[4] Fudan Univ, Shanghai Med Coll, Huadong Hosp, Dept Pulm Med, Shanghai 200433, Peoples R China

[5] Univ Auckland, Dept Anat Radiol, Auckland 1, New Zealand

来源：

RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY | 2011年 / 177卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Chronic obstructive pulmonary disease; Human lung fibroblasts; Transforming growth factor-beta 1; Proliferation; Elastin; GROWTH-FACTOR-BETA; LUNG FIBROBLASTS; GENE-EXPRESSION; EMPHYSEMA SHOW; MESSENGER-RNA; IN-VITRO; DISEASE; FIBERS; REPAIR; PROLIFERATION;

D O I：

10.1016/j.resp.2011.04.019

中图分类号：

Q4 [生理学];

学科分类号：

071003 [生理学];

摘要：

Insufficiency of tissue repair by pulmonary fibroblasts may contribute to the decrease in elastic fibres in chronic obstructive pulmonary disease (COPD). In this study, the repair function of COPD fibroblasts was assessed by examining the response to transforming growth factor (TGF)-beta 1. Primary pulmonary fibroblasts were cultured from lung tissue of COPD patients and smoking control subjects. Cellular proliferation was measured with Alamar Blue reduction method. Levels of tropoelastin mRNA and soluble elastin was measured using real-time RT-PCR and Fastin elastin assay respectively. The percentage of increase in proliferation and elastin production after TGF-beta 1 (1 ng/ml) treatment was calculated for fibroblasts from each subject. COPD fibroblasts showed slower proliferation than control fibroblasts, and a reduced response to TGF-beta 1 stimulation. The promotive effect of TGF-beta 1 on elastin synthesis in control fibroblasts was significantly diminished in fibroblasts from COPD patients. Our findings indicate that COPD lung fibroblasts have a significantly decreased response to TGF-beta 1 in terms of proliferation and elastin production. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：236 / 240

页数：5

共 26 条

[1]

The use of Alamar Blue assay for quantitative analysis of viability, migration and invasion of choriocarcinoma cells [J].