Flavonoids as inhibitors of NADP-malic enzyme and PEP carboxylase from C-4 plants

被引:18
作者
Pairoba, CF [1 ]
Colombo, SL [1 ]
Andreo, CS [1 ]
机构
[1] UNIV NACL ROSARIO, FAC CIENCIAS BIOQUIM & FARMACEUT, CTR ESTUDIOS FOTOSINETICOS & BIOQUIM, RA-2000 ROSARIO, ARGENTINA
关键词
flavonoid; quercetin; C-4; plant; NADP-malic enzyme; phosphoenolpyruvate carboxylase;
D O I
10.1271/bbb.60.779
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The inhibitory effects of flavonoids on the activity of two photosynthetic enzymes such as phosphoenolpyruvate carboxylase (PEPCase) and NADP-dependent malic enzyme (NADP-ME) were evaluated, The glycosylation of hydroxyl groups on the flavonoids resulted in compounds that behaved as gradually weaker inhibitors with increased size of the substituent, Quercetin and baicalein showed a competitive inhibition pattern vs, NADP(+) for NADP-ME, and a similar model for both flavonoids vs, phosphoenolpyruvate (PEP) was observed when tested on PEPCase, K-i for NADP-ME inhibition at pH 7.0 were 0.83 mu M and 1.54 mu M for quercetin and baicalein, respectively, K-i for PEPCase inhibition were 0.17 mu M and 0.79 mu M (quercetin and baicalein, respectively), indicating that these compounds are the most potent inhibitors described for this carboxylase. I-50 values for these and other flavonoids were in the micromolar range, A tentative physiological role for the inhibitory effects observed on PEPCase is discussed.
引用
收藏
页码:779 / 783
页数:5
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