A fast screening method for surface layers on Gram-positive bacteria

被引：9

作者：

Wahl, R

Raff, J

Selenska-Pobell, S

Mertig, M ^{[1
]}

Pompe, W

机构：

[1] Tech Univ Dresden, Inst Werkstoffwissensch, D-01069 Dresden, Germany

[2] Forschungszentrum Rossendorf EV, Inst Radiochem, D-01314 Dresden, Germany

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 18期

关键词：

Bacillaceae; bacterial surface layer (S layer); lysozyme; microscopy; screening;

D O I：

10.1023/A:1011637514787

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A two-step screening method is described to identify regularly arranged surface layers (S layers) on Gram-positive bacterial strains. A non-destructive release of S-layer sheets is achieved by enzymatic hydrolysis of the underlying peptidoglycan using lysozyme. The existence of regular S layers is then directly confirmed by scanning force microscopy or transmission electron microscopy. This method requires a minimal amount of bacterial cells and may be used as a `quick test' for demonstrating the presence of S layers.

引用

页码：1485 / 1490

页数：6

共 28 条

[1]

[Anonymous], 1996, Crystalline bacterial cell surface proteins

[2]

Baumeister W., 1987, ELECTRON MICROS, P109

[3] SURFACE ARRAYS ON THE WALL OF SPOROSARCINA-UREAE [J].