Properties of arabinonucleic acids (ANA & 20′F-ANA):: Implications for the design of antisense therapeutics that invoke RNase H cleavage of RNA

被引：25

作者：

Damha, MJ ^{[1
]}

Noronha, AM

Wilds, CJ

Trempe, JF

Denisov, A

Pon, RT

Gehring, K

机构：

[1] McGill Univ, Dept Chem, Montreal, PQ, Canada

[2] McGill Univ, Dept Biochem, Montreal, PQ, Canada

[3] Univ Calgary, Dept Biochem & Mol Biol, Calgary, AB, Canada

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2001年 / 20卷 / 4-7期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1081/NCN-100002317

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inversion of configuration of the C2' position of RNA leads to a very unique nucleic acid structure: arabinonucleic acid (ANA). ANA, and its 2'-fluoro derivative (2'F-ANA) form hybrids with RNA that are capable of activating RNase H, resulting in cleavage of the RNA strand. In this paper, we review the properties of duplexes formed between ANA (or 2'F-ANA) and its RNA complement. These studies support the notion that RNase H is sensitive to the minor groove dimensions of the hybrid substrate.

引用

页码：429 / 440

页数：12

共 46 条

[1] Significant inter- and intramolecular O-H•••FC hydrogen bonding [J].