Effects of dilated cardiomyopathy on the diaphragm in the Syrian hamster

This study aimed to elucidate changes in respiratory muscles and their mechanism in cardiomyopathy, The contractile properties and histology of the diaphragm, as well as serum levels of insulin-like growth factor (IGF)-1, were examined in 10 hamsters with idiopathic dilated cardiomyopathy (CM) and 10 controls. At 28 weeks, body weight in CM was reduced compared with controls (114+/-10 versus 144+/-14 g, p<0.0001), The ratio of diaphragm to body weight was significantly higher in CM than in controls (0.228+/-0.015 versus 0.182+/-0.017, p<0.0001), Dt vitro, maximal diaphragmatic twitch (303+/-63 versus 455+/-119 g.cm(-2)) and tetanic tensions (1,555+/-369 versus 2,204+/-506 g.cm(-2)) were significantly lower in CM than in controls (p<0.005). The half-relaxation time was significantly shorter in CM(19+/-1 ms) than in controls (24+/-3 ms, p<0.0005), Fatiguability at 25 Hz was significantly less in CM (28%) than in controls (42%, p<0.0001), Diaphragm and gastrocnemius adenosine triphosphatase staining showed type I fibre atrophy in CM, associated with an increase in the number of type I fibres in the diaphragm, Histological examination of both muscles revealed an abnormal muscular pattern, Finally, serum levels of IGF-1 were 47% lower in the CM group than in controls (p<0.0001) and were clearly related to the changes in the contractile properties and histology of the diaphragm, In conclusion, cardiomyopathy in hamsters: 1) depressed the force-generating capacity and shortened the relaxation of the hamster diaphragm; 2) induced type I fibre atrophy in combination with a myopathic pattern; and 3) was associated with a significant reduction in serum levels of insulin-like growth factor-1, related to the diaphragmatic changes. Whether these changes are primary myopathic or secondary to heart failure remains to be elucidated.