Sphingosine-1-phosphate mediates calcium signaling in guinea pig enteroglial cells

被引：15

作者：

Segura, BJ ^{[1
]}

Zhang, W ^{[1
]}

Xiao, L ^{[1
]}

Turner, D ^{[1
]}

Cowles, RA ^{[1
]}

Logsdon, C ^{[1
]}

Mulholland, MW ^{[1
]}

机构：

[1] Univ Michigan, Dept Surg, Ann Arbor, MI 48109 USA

来源：

JOURNAL OF SURGICAL RESEARCH | 2004年 / 116卷 / 01期

关键词：

sphingosine-1-phosphate; enteroglial cells; nervous system;

D O I：

10.1016/S0022-4804(03)00281-6

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

The enteric nervous system, which regulates multiple aspects of digestive activity, is composed of two major cell types, neurons and glial cells. Enteric glia, but not enteric neurons, respond to bioactive lipids with calcium signaling. The sphingomyelin metabolite sphingosine-l-phosphate (SIP) caused dose-dependent calcium (Ca2+) signaling using extracellular and intracellular Ca2+. The signal transduction cascade was pertussis toxin-insensitive and involved an extracellular receptor since repetitive exposure yielded diminished responsiveness. Inhibition of either phospholipase C or the inositol 1,4,5-trisphosphate'receptor abolished S1P effects. RT-PCR analysis demonstrated the presence of S1P-coupled endothelial differentiation gene (EDG) receptor mRNAs (EDG-1, EDG-3, and EDG-5) within the enteric nervous system. Immunocytochemical analysis demonstrated strong expression of both EDG-1 and EDG-3 and weak expression of EDG-5 in enteric glial cells. Other sphingomyelin cycle components, including sphingomyelin, sphingomyelinase, and sphingosine caused Ca2+ transients in enteric glia. Related lipids lysophosphatidic acid and sphingosylphosphorylcholine also induced Ca2+ signaling in enteric glia, suggesting that multiple lipid-activated signaling mechanisms exist in these cells. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：42 / 54

页数：13

共 38 条

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