Montanide™ ISA 71 VG adjuvant enhances antibody and cell-mediated immune responses to profilin subunit antigen vaccination and promotes protection against Eimeria acervulina and Eimeria tenella

被引：34

作者：

Jang, Seung I. ^{[1
,4
]}

Lillehoj, Hyun S. ^{[1
]}

Lee, Sung Hyen ^{[1
]}

Lee, Kyung Woo ^{[1
]}

Lillehoj, Erik P. ^{[2
]}

Bertrand, Francois ^{[3
]}

Dupuis, Laurent ^{[3
]}

Deville, Sebastien ^{[3
]}

机构：

[1] ARS, Anim Parasit Dis Lab, Anim & Nat Resources Inst, USDA, Beltsville, MD 20705 USA

[2] Univ Maryland, Sch Med, Dept Pediat, Baltimore, MD 21201 USA

[3] Seppic, F-92800 Puteaux La Defense, France

[4] Inst Hlth & Environm, Taejon 305338, South Korea

来源：

EXPERIMENTAL PARASITOLOGY | 2011年 / 127卷 / 01期

关键词：

Avian coccidiosis; Vaccine; CYTOKINE RESPONSES; COCCIDIOSIS; VACCINES; IMMUNIZATION; CHICKENS; PROTEIN; STRATEGIES; RESISTANCE; INFECTION; MAXIMA;

D O I：

10.1016/j.exppara.2010.07.021

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The present study was conducted to investigate the immunoenhancing effects of Montanide (TM) ISA 71 VG adjuvant on profilin subunit antigen vaccination. Broiler chickens were immunized subcutaneously with a purified Eimeria acervulina recombinant profilin protein, either alone or mixed with ISA 71 VG, and host immune responses were evaluated. After secondary immunization, antigen-specific antibody and T-cell responses were higher in the group which received profilin plus ISA 71 VG compared with the other groups. Furthermore, body weight gains and fecal oocyst shedding were evaluated following oral challenge infection with live E. acervulina or Eimeria tenella oocysts. Vaccination with profilin plus ISA 71 VG reduced oocyst shedding compared with animals immunized with profilin alone. These results demonstrate that the recombinant profilin subunit vaccine, when given in combination with Montanide (TM) ISA 71 VG, augments protective immunity against E. acervulina and E. tenella. Published by Elsevier Inc.

引用

页码：178 / 183

页数：6

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