Assessment of the lupin seed glucose-lowering protein intestinal absorption by using in vitro and ex vivo models

被引：27

作者：

Capraro, Jessica ^{[1
]}

Clemente, Alfonso ^{[2
]}

Rubio, Luis A. ^{[2
]}

Magni, Chiara ^{[1
]}

Scarafoni, Alessio ^{[1
]}

Duranti, Marcello ^{[1
]}

机构：

[1] Univ Milan, Dept AgriFood Mol Sci, I-20133 Milan, Italy

[2] CSIC, Estac Expt Zaidin, E-18008 Granada, Spain

来源：

FOOD CHEMISTRY | 2011年 / 125卷 / 04期

关键词：

Caco; 2; cells; gamma Conglutin; Intestinal everted sac; Intestinal uptake; Lupinus albus; GASTROINTESTINAL ABSORPTION; CONGLUTIN-GAMMA; AMINO-ACIDS; CACO-2; CELLS; ALBUS; TRANSPORT; LACTALBUMIN; MONOLAYERS; GLOBULINS; ALLERGENS;

D O I：

10.1016/j.foodchem.2010.10.073

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

A lupin seed glycoprotein termed gamma-conglutin has previously been found to display insulin mimetic activity in myocyte models and reduce plasma glucose concentration when orally administered to both rats and humans To envisage the possible metabolic fate of this bioactive protein we used in vitro cell and ex vivo tissue models to monitor its transit through the intestinal barrier Caco-2 cell monolayers and rat intestinal everted sacs were treated with purified gamma-conglutin and the protein was immuno assayed by chemi-luminescence-enhanced Western blotting The in vitro approach showed that the intact protein can transit from the apical to the basolateral side of the cell monolayers The unmodified lupin protein was also detected inside the intestinal everted sacs Proper controls of cell monolayer and sac integrity ruled out the possibility of protein passive leakage (C) 2010 Elsevier Ltd All rights reserved

引用

页码：1279 / 1283

页数：5

共 32 条

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