Maternal zinc deficiency reduces NMDA receptor expression in neonatal rat brain, which persists into early adulthood

被引:53
作者
Chowanadisai, W [1 ]
Kelleher, SL [1 ]
Lönnerdal, B [1 ]
机构
[1] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA
关键词
brain-derived neurotrophic factor; infant nutrition; nerve growth factor; neurotrophins; polysialic acid-neural cell adhesion molecule; postnatal development;
D O I
10.1111/j.1471-4159.2005.03246.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Prenatal and early postnatal zinc deficiency impairs learning and memory and these deficits persist into adulthood. A key modulator in this process may be the NMDA receptor; however, effects of zinc deficiency on the regulation of NMDA receptor activity are not well understood. Female Sprague-Dawley rats were fed diets containing 7 (zinc deficient, ZD), 10 (marginally zinc deficient, MZD) or 25 (control) mg Zn/g diet preconception through postnatal day (PN) 20, at which time pups were weaned onto their maternal or control diet. Regulation of NMDA receptor expression was examined at PN2, PN11, and PN65. At PN2, expression of whole brain NMDA receptor subunits NR1, NR2A, and NR2B was lower in pups from dams fed ZD and MZD compared to controls, as analyzed using relative RT-PCR and immunoblotting. At PN11, whole brain and hippocampi NR1, NR2A, NR2B and PSA-NCAM (polysialic acid-neural cell adhesion molecule) expression and the number of PSA-NCAM immunoreactive cells were lower in pups from dams fed ZD compared to controls. Whole brain brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) concentrations were lower in pups from dams fed ZD or both low zinc diets, respectively. Whole brain NR1 expression remained lower in previously zinc-deficient rats at PN65. These data indicate potential mechanisms through which developmental zinc deficiency can impair learning and memory later in life.
引用
收藏
页码:510 / 519
页数:10
相关论文
共 66 条
[1]   BDNF-triggered events in the rat hippocampus are required for both short- and long-term memory formation [J].
Alonso, M ;
Vianna, MRM ;
Depino, AM ;
Souza, TME ;
Pereira, P ;
Szapiro, G ;
Viola, H ;
Pitossi, F ;
Izquierdo, I ;
Medina, JH .
HIPPOCAMPUS, 2002, 12 (04) :551-560
[2]   DEVELOPMENT OF THE MOSSY FIBERS OF THE DENTATE GYRUS .1. A LIGHT AND ELECTRON-MICROSCOPIC STUDY OF THE MOSSY FIBERS AND THEIR EXPANSIONS [J].
AMARAL, DG ;
DENT, JA .
JOURNAL OF COMPARATIVE NEUROLOGY, 1981, 195 (01) :51-86
[3]   Zinc supplementation, mental development and behaviour in low birth weight term infants in northeast Brazil [J].
Ashworth, A ;
Morris, SS ;
Lira, PIC ;
Grantham-McGregor, SM .
EUROPEAN JOURNAL OF CLINICAL NUTRITION, 1998, 52 (03) :223-227
[4]   Zinc supplementation affects the activity patterns of rural Guatemalan infants [J].
Bentley, ME ;
Caulfield, LE ;
Ram, M ;
Santizo, MC ;
Hurtado, E ;
Rivera, JA ;
Ruel, MT ;
Brown, KH .
JOURNAL OF NUTRITION, 1997, 127 (07) :1333-1338
[5]   A SYNAPTIC MODEL OF MEMORY - LONG-TERM POTENTIATION IN THE HIPPOCAMPUS [J].
BLISS, TVP ;
COLLINGRIDGE, GL .
NATURE, 1993, 361 (6407) :31-39
[6]   MOLECULAR MACHINES INTEGRATE COINCIDENT SYNAPTIC SIGNALS [J].
BOURNE, HR ;
NICOLL, R .
CELL, 1993, 72 :65-75
[7]   Dual effects of NMDA receptor activation on polysialylated neural cell adhesion molecule expression during brainstem postnatal development [J].
Bouzioukh, F ;
Tell, F ;
Rougon, G ;
Jean, A .
EUROPEAN JOURNAL OF NEUROSCIENCE, 2001, 14 (08) :1194-1202
[8]   NMDA receptor and nitric oxide synthase activation regulate polysialylated neural cell adhesion molecule expression in adult brainstem synapses [J].
Bouzioukh, F ;
Tell, F ;
Jean, A ;
Rougon, G .
JOURNAL OF NEUROSCIENCE, 2001, 21 (13) :4721-4730
[9]   LOW ZINC STATUS IN GUINEA-PIGS IMPAIRS CALCIUM-UPTAKE BY BRAIN SYNAPTOSOMES [J].
BROWNING, JD ;
ODELL, BL .
JOURNAL OF NUTRITION, 1994, 124 (03) :436-443
[10]   ZINC-DEFICIENCY DECREASES THE CONCENTRATION OF N-METHYL-D-ASPARTATE RECEPTORS IN GUINEA-PIG CORTICAL SYNAPTIC-MEMBRANES [J].
BROWNING, JD ;
ODELL, BL .
JOURNAL OF NUTRITION, 1995, 125 (08) :2083-2089