Casiopeina III-ia induces apoptosis in HCT-15 cells in vitro through caspase-dependent mechanisms and has antitumor effect in vivo

被引：112

作者：

Carvallo-Chaigneau, Francisco ^{[1
]}

Trejo-Solis, Cristina ^{[2
]}

Gomez-Ruiz, Celedonio ^{[3
]}

Rodriguez-Aguilera, Ernesto ^{[4
]}

Macias-Rosales, Lucia ^{[3
]}

Cortes-Barberena, Edith ^{[4
]}

Cedillo-Pelaez, Carlos ^{[1
]}

Gracia-Mora, Isabel ^{[3
,5
]}

Ruiz-Azuara, Lena ^{[5
]}

Madrid-Marina, Vicente ^{[6
]}

Constantino-Casas, Fernando ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Fac Vet Med & Husbandry, Dept Pathol, Mexico City 04510, DF, Mexico

[2] SSA, Inst Neurol & Neurocirugia, Dept Neuroimmunol, Mexico City 14269, DF, Mexico

[3] Univ Nacl Autonoma Mexico, Fac Chem, Animal Expt Unit, Mexico City 04510, DF, Mexico

[4] Univ Autonoma Metropolitana Iztapalapa, Dept Hlth & Biol Sci, Mexico City 09340, DF, Mexico

[5] Univ Nacl Autonoma Mexico, Fac Chem, Dept Nucl & Inorgan Chem, Mexico City 04510, DF, Mexico

[6] Inst Nacl Salud Publ, Cuernavaca 62508, Morelos, Mexico

来源：

BIOMETALS | 2008年 / 21卷 / 01期

关键词：

casiopeina; apoptosis; chemotherapy; p53(-/-); Bax;

D O I：

10.1007/s10534-007-9089-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The aim of this study was to evaluate the in vitro and in vivo effects of the new chemotherapy agent Casiopeina III-ia [(4,4'-dimethyl-2,2'-bipiridine)(acetylacetonate) Copper (II) nitrate] on HCT-15 (p53-/-) colon cellular line. In vitro, the drug reduced the viability and induced necrosis and apoptosis in a dose dependent manner, without affecting cell cycle phases. Apoptosis was related to Bax increasing levels, suggesting a caspase-dependent mechanism of death, as verified by nucleosomal fragmentation of DNA. In vivo, the antitumor activity of Casiopeina III-ia was tested in HCT-15 cells transplanted to nude mice. In this study we will show that the novel antineoplastic agent Casiopeina III-ia is active on this colon tumor line, setting out as a good candidate for the treatment of colon tumors refractory to chemotherapy.

引用

页码：17 / 28

页数：12

共 40 条

[1]

MEASUREMENT OF DNA-EXCISION REPAIR IN SUSPENSIONS OF FRESHLY ISOLATED RAT HEPATOCYTES AFTER EXPOSURE TO SOME CARCINOGENIC COMPOUNDS - ITS POSSIBLE USE IN CARCINOGENICITY SCREENING [J].

BROUNS, RE ;

POOT, M ;

VRIND, RD ;

HOEKKON, TV ;

HENDERSON, PT ;

KUYPER, CMA .

MUTATION RESEARCH, 1979, 64 (06) :425-432

[2]

Biochemical pathways of caspase activation during apoptosis [J].

Budihardjo, I ;

Oliver, H ;

Lutter, M ;

Luo, X ;

Wang, XD .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1999, 15 :269-290

[3]

Disruption of p53 in human cancer cells alters the responses to therapeutic agents [J].

Bunz, F ;

Hwang, PM ;

Torrance, C ;

Waldman, T ;

Zhang, YG ;

Dillehay, L ;

Williams, J ;

Lengauer, C ;

Kinzler, KW ;

Vogelstein, B .

JOURNAL OF CLINICAL INVESTIGATION, 1999, 104 (03) :263-269

[4]

DNA damage-dependent acetylation of p73 dictates the selective activation of apoptotic target genes [J].

Costanzo, A ;

Merlo, P ;

Pediconi, N ;

Fulco, M ;

Sartorelli, V ;

Cole, PA ;

Fontemaggi, G ;

Fanciulli, M ;

Schiltz, L ;

Blandino, G ;

Balsano, C ;

Levrero, M .

MOLECULAR CELL, 2002, 9 (01) :175-186

[5]

Cvitkovic E, 1999, SEMIN ONCOL, V26, P647

[6]

Induction of apoptosis by a novel copper-based anticancer compound, casiopeina II, in L1210 murine leukaemia and CH1 human ovarian carcinoma cells [J].

De Vizcaya-Ruiz, A ;

Rivero-Muller, A ;

Ruiz-Ramirez, L ;

Kass, GEN ;

Kelland, LR ;

Orr, RM ;

Dobrota, M .

TOXICOLOGY IN VITRO, 2000, 14 (01) :1-5

[7]

DIZEREGA GS, 1994, FERTIL STERIL, V61, P219

[8]

FAN SJ, 1995, CANCER RES, V55, P1649

[9]

A novel mitochondriotoxic small molecule that selectively inhibits tumor cell growth [J].

Fantin, VR ;

Berardi, MJ ;

Scorrano, L ;

Korsmeyer, SJ ;

Leder, P .

CANCER CELL, 2002, 2 (01) :29-42

[10]

Control of apoptosis by p53 [J].

Fridman, JS ;

Lowe, SW .

ONCOGENE, 2003, 22 (56) :9030-9040

← 1 2 3 4 →