β-Actin specifically controls cell growth, migration, and the G-actin pool

被引：266

作者：

Bunnell, Tina M. ^{[1
]}

Burbach, Brandon J. ^{[2
]}

Shimizu, Yoji ^{[2
]}

Ervasti, James M. ^{[1
]}

机构：

[1] Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA

[2] Univ Minnesota, Sch Med, Dept Lab Med & Pathol, Ctr Immunol,Masonic Canc Ctr, Minneapolis, MN 55414 USA

来源：

MOLECULAR BIOLOGY OF THE CELL | 2011年 / 22卷 / 21期

基金：

美国国家卫生研究院;

关键词：

SERUM RESPONSE FACTOR; MESSENGER-RNA; GAMMA-ACTIN; SIGNAL-TRANSDUCTION; CULTURED-CELLS; NUCLEAR ACTIN; EXPRESSION; MOTILITY; CYTOSKELETON; GENE;

D O I：

10.1091/mbc.E11-06-0582

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Ubiquitously expressed beta-actin and gamma-actin isoforms play critical roles in most cellular processes; however, their unique contributions are not well understood. We generated whole-body beta-actin-knockout (Actb(-/-)) mice and demonstrated that beta-actin is required for early embryonic development. Lethality of Actb(-/-) embryos correlated with severe growth impairment and migration defects in beta-actin-knockout primary mouse embryonic fibroblasts (MEFs) that were not observed in gamma-actin-null MEFs. Migration defects were associated with reduced membrane protrusion dynamics and increased focal adhesions. We also identified migration defects upon conditional ablation of beta-actin in highly motile T cells. Of great interest, ablation of beta-actin altered the ratio of globular actin (G-actin) to filamentous actin in MEFs, with corresponding changes in expression of genes that regulate the cell cycle and motility. These data support an essential role for beta-actin in regulating cell migration and gene expression through control of the cellular G-actin pool.

引用

页码：4047 / 4058

页数：12

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