PCR for the identification of methicillin-resistant Staphylococcus aureus (MRSA) strains using a single primer pair specific for SCCmec elements and the neighbouring chromosome-borne orfX

被引：43

作者：

Cuny, C ^{[1
]}

Witte, W ^{[1
]}

机构：

[1] Robert Koch Inst, Wernigerode Branch, D-38855 Wernigerode, Germany

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2005年 / 11卷 / 10期

关键词：

methicillin-resistant Staphylococcus aureus; MRSA; orfX; PCR; typing; SCCmec;

D O I：

10.1111/j.1469-0691.2005.01236.x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The chromosomal location of the SCCmec elements containing mecA allows the identification of methicillin-resistant Staphylococcus aureus (MRSA) strains by PCR amplification of a sequence covering the right junction of the SCCmec elements and the adjacent chromosomal region encoding the species-specific ORFX. MRSA strains can be identified specifically using one forward primer, with only one or two mismatches, targeting the SCCmec elements of different types, and one reverse primer targeting the orfX region.

引用

页码：834 / 837

页数：4

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