Effect of testosterone administration and weight training on muscle architecture

Purpose: The purpose of this study was to assess muscle architecture changes in subjects who were administered supraphysiologic doses of testosterone enanthate (TE) and concurrently performed heavy resistance training. Methods: Ten subjects were randomly selected from the 21 subjects who participated in a previously published study (12). Subjects were allocated to one of two groups as per Giorgi et al. (12) and received either a saline-based placebo (nonTE) or a 3.5-mg.kg(-1) body weight dose of TE by deep intramuscular injection once a week for 12 wk. Subjects also performed heavy resistance training using exercises that targeted the triceps brachii muscle. Before and after the training period, free-weight one-repetition-maximum (1-RM) bench press strength was tested, muscle thickness and pennation of the triceps brachii lateralis were measured using ultrasound imaging, and fascicle length was estimated from ultrasound photographs. Results: There were no significant between-group differences in muscle thickness changes despite a trend toward increased thickness in TE subjects (TE, 23.5%, vs nonTE, 13.8%). However, 1-RM bench press performance and muscle pennation increased significantly in TE subjects compared with nonTE subjects (P < 0.05). There was also a trend toward longer fascicle lengths in the muscles of nonTE subjects. Conclusion: The results of the present study suggest that the use of TE in conjunction with heavy resistance training is associated with muscle architecture changes that are commonly associated with high-force production. Since there was little difference between the groups in muscle thickness, changes in pennation and possibly fascicle length may have contributed to strength gains seen in TE subjects.