The nature of the bond between peptide and carrier molecule determines the immunogenicity of the construct

被引：9

作者：

Beekman, NJCM ^{[1
]}

Schaaper, WMM ^{[1
]}

Langeveld, JPM ^{[1
]}

Boshuizen, RS ^{[1
]}

Meloen, RH ^{[1
]}

机构：

[1] Inst Anim Sci & Hlth ID Lelystad, Dept Mol Recognit, NL-8200 AB Lelystad, Netherlands

来源：

JOURNAL OF PEPTIDE RESEARCH | 2001年 / 58卷 / 03期

关键词：

carrier molecule; immunogenicity; labile bond; lipo peptide; palmitic acid; thioester; vaccine;

D O I：

10.1034/j.1399-3011.2001.00905.x

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence of the nature of the bond between a peptide and a (lipidic) carrier molecule on the immunogenicity of that construct was investigated. As types of bonds a thioester-, a disulfide-, an amide- and a thioether bond were investigated. As carrier molecules a peptide, an N-palmitoylated peptide or a C-16-hydrocarbon chain were used. The biostability of the bond between peptide and carrier molecule is thioether > amide > disulfide much greater than thioester. However, the immunogenic potency of the constructs used was found to be thioester > disulfide > amide > thioether. In conclusion, a construct with a bond between peptide and (lipidic) carrier molecule that is more susceptible to biological degradation is more immunogenic when used in a peptide-based vaccine than a bond that is less susceptible to biological degradation.

引用

页码：237 / 245

页数：9

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