Evaluation of Eudragit-coated chitosan microparticles as an oral immune delivery system

被引:58
作者
Hori, M [1 ]
Onishi, H [1 ]
Machida, Y [1 ]
机构
[1] Hoshi Univ, Dept Drug Delivery Res, Shinagawa Ku, Tokyo 1428501, Japan
关键词
chitosan microparticle; oral vaccine; ovalbumin; Eudragit L100; immune response;
D O I
10.1016/j.ijpharm.2005.04.008
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Chitosan microparticles containing ovalbumin (OVA), OVA-containing chitosan microparticles (Chi-OVA), were prepared, coated with Eudragit L 100 (ER), and evaluated as oral vaccine. Chi-OVA with an OVA content of 34.4% (w/w) and a mean particle size of 2.3 mu m were used for experiments in vitro and in vivo. ER-coated Chi-OVA (ER-Chi-OVA) contained 3.6-20.5% (w/w) OVA and had a particle size of 47.9-161.1 mu m. Chi-OVA dissolved readily in JP 14 first fluid, but not in JP 14 second fluid. The release of OVA from Chi-OVA was suppressed extensively in JP 14 second fluid. ER-Chi-OVA did not dissolve in JP 14 first fluid, and the release of OVA was suppressed greatly in JP 14 first and second fluids. OVA solution, Chi-OVA and ER-Chi-OVA (200 and 800 mu g OVA/mouse) were administered to Balb/C mice twice at a 1-week interval. At 7 d after the second administration, plasma OVA-specific IgG and fecal OVA-specific IgA levels were measured. OVA-specific IgG tended to be enhanced in Chi-OVA and ER-Chi-OVA, but was the highest in OVA solution. OVA-specific IgA was induced significantly more efficiently by ER-Chi-OVA than the others. These suggested that ER-Chi-OVA should be possibly useful to induce an intestinal mucosal immune response. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:223 / 234
页数:12
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