Assessment of efflux pump gene expression in a clinical isolate Mycobacterium tuberculosis by real-time reverse transcription PCR

被引：76

作者：

Jiang, Xin ^{[1
,2
]}

Zhang, Wenhong ^{[3
]}

Zhang, Ying ^{[4
]}

Gao, Feng ^{[5
]}

Lu, Chanyi ^{[2
]}

Zhang, Xuelian ^{[2
]}

Wang, Honghai ^{[2
]}

机构：

[1] Fudan Univ, Huashan Hosp, Shanghai 200433, Peoples R China

[2] Shanghai Univ TCM, Shanghai, Peoples R China

[3] Fudan Univ, Sch Life Sci, Inst Genet, State Key Lab Genet Engn, Shanghai 200433, Peoples R China

[4] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Mol Microbiol & Immunol, Baltimore, MD USA

[5] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 1, Shanghai 200030, Peoples R China

来源：

MICROBIAL DRUG RESISTANCE | 2008年 / 14卷 / 01期

基金：

英国惠康基金; 英国工程与自然科学研究理事会;

关键词：

D O I：

10.1089/mdr.2008.0772

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The importance of some putative efflux pumps in conferring drug resistance on certain strain of Mycobacterium tuberculosis was determined by using multidrug-resistant (MDR) strain 1499 bearing defined mutations in rpoB and katG. The clinical isolate 1499 was tested for drug sensitivity and expression level of five putative efflux pump genes by real-time RT-PCR. In the presence of rifampicin or isoniazid, this isolate showed increased transcription of putative efflux pump genes Rv1258c and Rv1410c. Rv1819c was overexpressed only upon isoniazid exposure (p<0.05). Overexpression of some efflux genes is associated with multidrug resistance in certain clinical isolate of M. tuberculosis.

引用

页码：7 / 11

页数：5

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