Microbial life in champagne pool, a geothermal spring in Waiotapu, New Zealand

被引:44
作者
Hetzer, Adrian
Morgan, Hugh W.
McDonald, Ian R.
Daughney, Christopher J.
机构
[1] Univ Waikato, Thermophile Res Unit, Hamilton 3240, New Zealand
[2] Univ Waikato, Dept Biol Sci, Hamilton 3240, New Zealand
[3] Inst Geol & Nucl Sci, Lower Hutt, New Zealand
关键词
champagne Pool; Waiotapu; geothermal; thermophilic microorganisms; hydrogen-oxidizer; sulfate-reducer; arsenic;
D O I
10.1007/s00792-007-0073-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Surveys of Champagne Pool, one of New Zealand's largest terrestrial hot springs and rich in arsenic ions and compounds, have been restricted to geological and geochemical descriptions, and a few microbiological studies applying culture-independent methods. In the current investigation, a combination of culture and culture-independent approaches were chosen to determine microbial density and diversity in Champagne Pool. Recovered total DNA and adenosine 5'-triphosphate (ATP) content of spring water revealed relatively low values compared to other geothermal springs within New Zealand and are in good agreement with low cell numbers of 5.6 +/- 0.5 x 10(6) cells/ml obtained for Champagne Pool water samples by 4',6-diamidino-2-phenylindole (DAPI) staining. Denaturing Gradient Gel Electrophoresis (DGGE) and 16S rRNA (small-subunit ribosomal nucleic acid) gene clone library analyses of environmental DNA indicated the abundance of Sulfurihydrogenibium, Sulfolobus, and Thermofilum-like populations in Champagne Pool. From these results, media were selected to target the enrichment of hydrogen-oxidizing and sulfur-dependent microorganisms. Three isolates were successfully obtained having 16S rRNA gene sequences with similarities of similar to 98% to Thermoanaerobacter tengcongensis, 94% to Sulfurihydrogenibium azorense, and 99% to Thermococcus waiotapuensis, respectively.
引用
收藏
页码:605 / 614
页数:10
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