Sex determination in beef by melting curve analysis of PCR amplicons from the amelogenin locus

被引：18

作者：

Ballin, Nicolal Z. ^{[1
]}

Madsen, Knud G. ^{[1
]}

机构：

[1] Danish Vet & Food Adm, Dept Food Chem Reg Vet & Food Control Authorit, DK-4100 Ringsted, Denmark

来源：

MEAT SCIENCE | 2007年 / 77卷 / 03期

关键词：

real time PCR; beef; cattle; sex determination; amelogenin gene; melting curve analysis;

D O I：

10.1016/j.meatsci.2007.04.029

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Sex determination of beef is important to meet the rules of the Commission Regulation (EC) 765/2002 that qualify for export refunds. A SYBR Green sex identification assay based on melting curve analysis of PCR amplicons from the amelogenin locus (AMELX and AMELY) was developed. The PCR amplicons of 130/130 and 130/67 base pairs produced from female and male beef, respectively, are easily distinguished by both melting curve analysis and gel electrophoresis. Results from the melting curve analysis of amplicons are ready in less than three minutes, and requires no additional work in addition to the PCR setup. Applicability of the sex determination assay was studied by analysis of 12 unknown beef samples and the results were compared to an accredited method based on gel electrophoresis. In addition, six different cattle breeds were examined. All test results were correct in respect to sex. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：384 / 388

页数：5

共 15 条

[1] Prediction of X and Y chromosome content in bovine sperm by using DNA pools through capillary electrophoresis
Checa, ML
Dunner, S
Cañón, J
[J]. THERIOGENOLOGY, 2002, 58 (08) : 1579 - 1586
[2] Chen CM, 1999, MOL REPROD DEV, V54, P209, DOI 10.1002/(SICI)1098-2795(199911)54:3&lt
[3] 209::AID-MRD1&gt
[4] 3.0.CO
[5] 2-6
[6] A PCR-BASED SEX-DETERMINATION ASSAY IN CATTLE BASED ON THE BOVINE AMELOGENIN LOCUS
ENNIS, S
GALLAGHER, TF
[J]. ANIMAL GENETICS, 1994, 25 (06) : 425 - 427
[7] STRUCTURE AND EXPRESSION OF THE BOVINE AMELOGENIN GENE
GIBSON, C
GOLUB, E
HEROLD, R
RISSER, M
DING, W
SHIMOKAWA, H
YOUNG, M
TERMINE, J
ROSENBLOOM, J
[J]. BIOCHEMISTRY, 1991, 30 (04) : 1075 - 1079
[8] KIRKPATRICK BW, 1993, J REPROD FERTIL, V98, P335, DOI 10.1530/jrf.0.0980335
[9] MACHATY Z, 1993, J REPROD FERTIL, V98, P467, DOI 10.1530/jrf.0.0980467
[10] Sex ratio determination in bovine semen: A new approach by quantitative real time PCR
Parati, K.
Bongioni, G.
Aleandri, R.
Galli, A.
[J]. THERIOGENOLOGY, 2006, 66 (09) : 2202 - 2209

← 1 2 →