Human periodontal ligament cell sheets can regenerate periodontal ligament tissue in an athymic rat model

被引:203
作者
Hasegawa, M
Yamato, M
Kikuchi, A
Okano, T
Ishikawa, I
机构
[1] Tokyo Med & Dent Univ, Grad Sch, Dept Hard Tissue Engn, Sect Periodontol,Bunkyo Ku, Tokyo 1138549, Japan
[2] Tokyo Med & Dent Univ, Ctr Excellence rogram Frontier Res Mol Destruct &, Tokyo 1138549, Japan
[3] Tokyo Womens Med Univ, Inst Adv Biomed Engn & Sci, Tokyo, Japan
[4] Tokyo Womens Med Univ, Ctr Excellence Program Regenerat Med, Tokyo, Japan
来源
TISSUE ENGINEERING | 2005年 / 11卷 / 3-4期
关键词
D O I
10.1089/ten.2005.11.469
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Conventional periodontal regeneration methods remain insufficient to attain complete and reliable clinical regeneration of periodontal tissues. We have developed a new method of cell transplantation using cell sheet engineering and have applied it to this problem. The purpose of this study was to investigate the characteristics of human periodontal ligament (HPDL) cell sheets retrieved from culture on unique temperature-responsive culture dishes, and to examine whether these cell sheets can regenerate periodontal tissues. The HPDL cell sheets were examined histologically and biochemically, and also were transplanted into a mesial dehiscence model in athymic rats. HPDL cells were harvested from culture dishes as a contiguous cell sheet with abundant extracellular matrix and retained intact integrins that are susceptible to trypsin-EDTA treatment. In the animal study, periodontal ligament-like tissues that include an acellular cementum-like layer and fibrils anchoring into this layer were identified in all the athymic rats transplanted with HPDL cell sheets. This fibril anchoring highly resembles native periodontal ligament fibers; such regeneration was not observed in nontransplanted controls. These results suggest that this technique, based on the concept of cell sheet engineering, can be useful for periodontal tissue regeneration.
引用
收藏
页码:469 / 478
页数:10
相关论文
共 43 条
[1]   Tissue engineering: a new paradigm for periodontal regeneration based on molecular and cell biology [J].
Bartold, PM ;
McCulloch, CAG ;
Narayanan, AS ;
Pitaru, S .
PERIODONTOLOGY 2000, 2000, 24 :253-269
[2]   The periodontal ligament: A unique, multifunctional connective tissue [J].
Beertsen, W ;
McCulloch, CAG ;
Sodek, J .
PERIODONTOLOGY 2000, 1997, 13 :20-40
[3]  
BUSER D, 1990, International Journal of Oral and Maxillofacial Implants, V5, P113
[4]   Development and general structure of the periodontium [J].
Cho, MI ;
Garant, PR .
PERIODONTOLOGY 2000, 2000, 24 :9-27
[5]  
Choi BH, 2000, INT J ORAL MAX IMPL, V15, P193
[6]   Expression of bone associated markers by tooth root lining cells, in situ and in vitro [J].
DErrico, JA ;
Macneil, RL ;
Takata, T ;
Berry, J ;
Strayhorn, C ;
Somerman, MJ .
BONE, 1997, 20 (02) :117-126
[7]   Assessment of periodontal healing by seeding of fibroblast-like cells derived from regenerated periodontal ligament in artificial furcation defects in a dog:: A pilot study [J].
Dogan, A ;
Özdemir, A ;
Kubar, A ;
Oygür, T .
TISSUE ENGINEERING, 2002, 8 (02) :273-282
[8]   COMPOSITE JAW AND ILIAC CANCELLOUS BONE-GRAFTS IN INTRABONY DEFECTS IN MONKEYS [J].
ELLEGAARD, B ;
NIELSEN, IM ;
KARRING, T .
JOURNAL OF PERIODONTAL RESEARCH, 1976, 11 (05) :299-310
[9]   NEW ATTACHMENT FORMATION AS THE RESULT OF CONTROLLED TISSUE REGENERATION [J].
GOTTLOW, J ;
NYMAN, S ;
KARRING, T ;
LINDHE, J .
JOURNAL OF CLINICAL PERIODONTOLOGY, 1984, 11 (08) :494-503
[10]   L-ASCORBIC-ACID 2-PHOSPHATE STIMULATES COLLAGEN ACCUMULATION, CELL-PROLIFERATION, AND FORMATION OF A 3-DIMENSIONAL TISSUE-LIKE SUBSTANCE BY SKIN FIBROBLASTS [J].
HATA, RI ;
SENOO, H .
JOURNAL OF CELLULAR PHYSIOLOGY, 1989, 138 (01) :8-16