Cleavage of cystatin C is not associated with multiple sclerosis

被引：29

作者：

Del Boccio, Piero

Pieragostino, Damiana

Lugaresi, Alessandra

Di Ioia, Maria

Pavone, Barbara

Travaglini, Daniela

D'Aguanno, Simona

Bernardini, Sergio

Sacchetta, Paolo

Federici, Giorgio

Di Ilio, Carmine

Gambi, Domenico

Urbani, Andrea

机构：

[1] Univ G dAnnunzio, Ctr Excellence Aging, CeSI, Fdn G Dannunzio, I-66100 Chieti, Italy

[2] Univ G Dannunzio Chieti & Pescare, Dipartimento Sci BioMed, Chieti, Italy

[3] IRCCS, Fdn S Lucia, Ctr Europeo Ric Cervello, Rome, Italy

[4] Univ G Dannunzio Chieti & Pescare, Dipartimento Oncol & Neurosci, Chieti, Italy

[5] Univ Roma Tor Vergata, Dipartimento Med Interna, Rome, Italy

来源：

ANNALS OF NEUROLOGY | 2007年 / 62卷 / 02期

关键词：

D O I：

10.1002/ana.20968

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Recently, Irani and colleagues proposed a C-terminal cleaved isoform cystatin C (12.5kDa) in cerebrospinal fluid as a marker of multiple sclerosis. In this study, we demonstrate that the 12.5kDa product of cystatin C is formed by degradation of the first eight N-terminal residues. Moreover, such a degradation is not specific in the cerebrospinal fluid of multiple sclerosis, but rather is given by an inappropriate sample storage at -20 degrees C. We conclude that the use of the 12.5kDa product of cystatin C in cerebrospinal fluid might lead to a fallacious diagnosis of multiple sclerosis. Preanalytical validation procedure is mandatory for proteomics investigations.

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页码：201 / 204

页数：4

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