Multiple Y receptors mediate pancreatic polypeptide responses in mouse colon mucosa

A functional study has been performed to characterise the Y receptors responsible for NPY, PYY and PP-stimulated responses in mouse colonic mucosal preparations. Electrogenic ion secretion was stimulated with VIP following which NPY, PYY and PP analogues were, to varying degrees, inhibitory. PYY(3-36), hPP, Gln(23)hPP and rPP were effective but less potent than full length PYY, NPY or their Pro(34)-substituted analogues, while the Y-5 agonist Ala(31), Aib(32)hNPY was the least active peptide tested. The Y-1 antagonists, BIBP3226 and BIBO3304 virtually abolished Pro(34)PYY and PYY responses while PYY(3-36) responses were selectively inhibited by the Y-5 antagonist, BIIE0246. A combination of BIBO3301 and BIIE0246 also partially attenuated hPP responses, leaving residual effects that were most probably Y-4-mediated. Thus we conclude that Y-1, Y-2 and Y-4 receptors attenuate ion secretion in mouse colon. (C) 2001 Elsevier Science Inc. All rights reserved.