Chemical identification of N-acyl homoserine lactone quorum-sensing signals produced by Sinorhizobium meliloti strains in defined medium

被引：58

作者：

Teplitski, M

Eberhard, A

Gronquist, MR

Gao, M

Robinson, JB

Bauer, WD

机构：

[1] Ohio State Univ, Dept Hort & Crop Sci, Columbus, OH 43210 USA

[2] Cornell Univ, Dept Microbiol, Ithaca, NY 14853 USA

[3] SUNY Coll Cortland, Dept Chem, Cortland, NY 13045 USA

[4] Univ Dayton, Dept Biol, Dayton, OH 45460 USA

来源：

ARCHIVES OF MICROBIOLOGY | 2003年 / 180卷 / 06期

关键词：

quorum sensing; Sinorhizobium; N-acyl homoserine lactones; mass spectrometry;

D O I：

10.1007/s00203-003-0612-x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The N-acyl homoserine lactone (AHL) quorum-sensing signals produced by Sinorhizobium meliloti strains AK631 and 1021 when cultured in a defined glucose-nitrate medium were identified by gas chromatography/mass spectrometry (GC/MS) and electrospray ionization tandem mass spectrometry (ESI MS/MS). Both strains synthesized several long-chain AHLs. Defined medium cultures of strain AK631 synthesized a complex mixture of AHLs with short acyl side chains. Strain 1021 produced no short-chain AHLs when grown on defined medium and made a somewhat different set of long-chain AHLs than previously reported for cultures in rich medium. While the two strains produced several AHLs in common, the differences in AHLs produced suggest that there may be significant differences in their patterns of quorum-sensing regulation.

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收藏

页码：494 / 497

页数：4

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