The effects and mechanisms of clinorotation on proliferation and differentiation in bone marrow mesenchymal stem cells

被引：43

作者：

Yan, Ming ^{[1
]}

Wang, Yongchun ^{[2
]}

Yang, Min ^{[1
]}

Liu, Yanwu ^{[1
]}

Qu, Bo ^{[3
]}

Ye, Zhengxu ^{[1
]}

Liang, Wei ^{[1
]}

Sun, Xiqing ^{[2
]}

Luo, Zhuojing ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Dept Orthopaed Surg, XiJing Hosp, Xian 710032, Peoples R China

[2] Fourth Mil Med Univ, Sch Aerosp Med, Dept Aerosp Biodynam, Xian 710032, Peoples R China

[3] Chengdu Mil Gen Hosp, Chengdu 610083, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2015年 / 460卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Simulated microgravity; BMSCs; Proliferation; Differentiation; SATB2; STROMAL CELLS; MODELED MICROGRAVITY; GENE-EXPRESSION; SPACEFLIGHT; RESORPTION; PROTEINS; EXPOSURE; MARKERS; FUTURE;

D O I：

10.1016/j.bbrc.2015.03.034

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Data from human and rodent studies have demonstrated that microgravity induces observed bone loss in real spaceflight or simulated experiments. The decrease of bone formation and block of maturation may play important roles in bone loss induced by microgravity. The aim of this study was to investigate the changes of proliferation and differentiation in bone marrow mesenchymal stem cells (BMSCs) induced by simulated microgravity and the mechanisms underlying it. We report here that clinorotation, a simulated model of microgravity, decreased proliferation and differentiation in BMSCs after exposure to 48 h simulated microgravity. The inhibited proliferation are related with blocking the cell cycle in G2/M and enhancing the apoptosis. While alterations of the osteoblast differentiation due to the decreased SATB2 expression induced by simulated microgravity in BMSCs. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：327 / 332

页数：6

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