Amplification of cDNA ends based on template-switching effect and step-out PCR

被引：318

作者：

Matz, M

Shagin, D

Bogdanova, E

Britanova, O

Lukyanov, S

Diatchenko, L

Chenchik, A

机构：

[1] Clontech Labs Inc, Palo Alto, CA 94303 USA

[2] Russian Acad Sci, Inst Bioorgan Chem, Moscow 117871, Russia

来源：

NUCLEIC ACIDS RESEARCH | 1999年 / 27卷 / 06期

关键词：

D O I：

10.1093/nar/27.6.1558

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to amplify flanking sequences of any cDNA species present in the sample.

引用

页码：1558 / 1560

页数：3

共 6 条

[1] The elimination of primer-dimer accumulation in PCR [J].