Nested polymerase chain reaction for detection of Ehrlichia risticii genomic DNA in infected horses

被引：31

作者：

Barlough, JE ^{[1
]}

Rikihisa, Y ^{[1
]}

Madigan, JE ^{[1
]}

机构：

[1] OHIO STATE UNIV,COLL VET MED,DEPT VET BIOSCI,COLUMBUS,OH 43210

来源：

VETERINARY PARASITOLOGY | 1997年 / 68卷 / 04期

关键词：

Ehrlichia risticii; Ixodes pacificus; equine monocytic ehrlichiosis; Potomac horse fever; horse; diagnosis; Rickettsia; polymerase chain reaction; southern hybridization test; DNA sequence analysis;

D O I：

10.1016/S0304-4017(96)01083-7

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A nested polymerase chain reaction was developed for amplifying a 529-bp segment of the 16S ribosomal RNA gene of Ehrlichia risticii from equine buffy coat cells. Confirmation of identity of the amplified bands was accomplished by Southern hybridization and DNA sequencing. The study indicated a detection limit of > 10 copies of the target gene, and specificity for E. risticii as based on a panel of test rickettsiae. Ticks (Ixodes pacificus) collected in an area of northern California enzootic for equine monocytic ehrlichiosis were found to be negative for E. risticii DNA.

引用

页码：367 / 373

页数：7

共 16 条

[1] Nested polymerase chain reaction for detection of Ehrlichia equi genomic DNA in horses and ticks (Ixodes pacificus) [J].

Barlough, JE ;

Madigan, JE ;

DeRock, E ;

Bigornia, L .

VETERINARY PARASITOLOGY, 1996, 63 (3-4) :319-329

[2] PHYLOGENY OF HARD-TICK AND SOFT-TICK TAXA (ACARI, IXODIDA) BASED ON MITOCHONDRIAL 16S RDNA SEQUENCES [J].

BLACK, WC ;

PIESMAN, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (21) :10034-10038

[3]

Chaichanasiriwithaya W., 1994, J CLIN MICROBIOL, V38, P3026

[4] ATTEMPTED TRANSMISSION OF EHRLICHIA-RISTICII, CAUSATIVE AGENT OF POTOMAC HORSE FEVER, BY THE TICKS, DERMACENTOR-VARIABILIS, RHIPICEPHALUS-SANGUINEUS, IXODES-SCAPULARIS AND AMBLYOMMA-AMERICANUM [J].

HAHN, NE ;

FLETCHER, M ;

RICE, RM ;

KOCAN, KM ;

HANSEN, JW ;

HAIR, JA ;

BARKER, RW ;

PERRY, BD .

EXPERIMENTAL & APPLIED ACAROLOGY, 1990, 8 (1-2) :41-50

[5]

KAHLER S, 1989, J AM VET MED ASSOC, V194, P1681

[6]

MADIGAN JE, 1995, J AM VET MED ASSOC, V207, P1448

[7] STUDIES ON ORAL-TRANSMISSION OF POTOMAC HORSE FEVER [J].

PALMER, JE ;

BENSON, CE .

JOURNAL OF VETERINARY INTERNAL MEDICINE, 1994, 8 (02) :87-92

[8] POTOMAC HORSE FEVER [J].

PALMER, JE .

VETERINARY CLINICS OF NORTH AMERICA-EQUINE PRACTICE, 1993, 9 (02) :399-410

[9] 16S RIBOSOMAL-RNA GENE SEQUENCE OF NEORICKETTSIA-HELMINTHOECA AND ITS PHYLOGENETIC ALIGNMENT WITH MEMBERS OF THE GENUS EHRLICHIA [J].

PRETZMAN, C ;

RALPH, D ;

STOTHARD, DR ;

FUERST, PA ;

RIKIHISA, Y .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1995, 45 (02) :207-211

[10] CAUSATIVE EHRLICHIAL ORGANISMS IN POTOMAC HORSE FEVER [J].

RIKIHISA, Y ;

PERRY, BD .

INFECTION AND IMMUNITY, 1985, 49 (03) :513-517

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