Do-it-yourself guide: how to use the modern single-molecule toolkit

被引：244

作者：

Walter, Nils G. ^{[1
]}

Huang, Cheng-Yen ^{[1
]}

Manzo, Anthony J. ^{[1
]}

Sobhy, Mohamed A. ^{[1
]}

机构：

[1] Univ Michigan, Dept Chem, Single Mol Anal Grp, Ann Arbor, MI 48109 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 06期

关键词：

D O I：

10.1038/nmeth.1215

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Single-molecule microscopy has evolved into the ultimate-sensitivity toolkit to study systems from small molecules to living cells, with the prospect of revolutionizing the modern biosciences. Here we survey the current state of the art in single-molecule tools including fluorescence spectroscopy, tethered particle microscopy, optical and magnetic tweezers, and atomic force microscopy. We also provide guidelines for choosing the right approach from the available single-molecule toolkit for applications as diverse as structural biology, enzymology, nanotechnology and systems biology.

引用

页码：475 / 489

页数：15

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