Measurement of nicotine in hair by reversed-phase highperformance liquid chromatography with electrochemical detection

被引：72

作者：

Mahoney, GN

Al-Delaimy, W

机构：

[1] Capital Coast Hlth Ltd, Lab Serv, Wellington, New Zealand

[2] Wellington Sch Med, Dept Publ Hlth, Wellington, New Zealand

来源：

JOURNAL OF CHROMATOGRAPHY B | 2001年 / 753卷 / 02期

关键词：

nicotine;

D O I：

10.1016/S0378-4347(00)00540-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed an assay for nicotine in hair based on reversed-phase HPLC with electrochemical detection. The method uses a low-metal, high-purity silica reversed-phase column. We have investigated the washing, digestion and extraction procedures and discuss the important points in the HPLC method development. The assay is presented as an application in a population of exposed and non-exposed children. Analytical parameters are satisfactory with linearity, recoveries, limit of quantitation and precision all suitable for epidemiological studies involving environmental tobacco smoke exposure assessment. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：179 / 187

页数：9

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[1] SAMPLE PREPARATION TECHNIQUES [J].