Inhibition of proximal convoluted tubule transport by dopamine

Background Dopamine can produce a natriuresis and diuresis independent of changes in renal hemodynamics. However, previous studies have failed to demonstrate an inhibition of transport by dopamine in intact proximal convoluted tubules. Methods. Rabbit proximal convoluted tubules were perfused in vitro with an ultrafiltrate-like solution and bathed in a serum-like albumin solution. Results. In the present study, the addition of 10(-5) M dopamine to the lumen or bath of proximal convoluted tubules perfused in vitro had no effect on transport. In proximal convoluted tubules, addition of 10(-6) M bath norepinephrine increased the rate of volume absorption from 0.65 +/- 0.08 to 0.93 +/- 0.08 nl/mm . min (P < 0.01). Addition of 10(-5) M luminal dopamine in the presence of bath norepinephrine inhibited the rate of volume absorption to 0.72 +/- 0.10 nl/mm . min (P = 0.01). The inhibition in the rate of volume absorption by luminal dopamine in the presence of bath norepinephrine was completely blocked by the DA, antagonist, SCH 23390. The DA(1) agonist luminal 10(-5) M fenoldopam also inhibited volume absorption in the presence of bath norepinephrine, but the DA, agonist luminal 10(-5) M quinpirole was without effect. Bath 10(-5) M dopamine had no effect on volume absorption in the presence of bath norepinephrine. Conclusion. Dopamine has no direct epithelial action on the proximal convoluted tubule. However, luminal dopamine antagonizes the stimulation in transport produced by norepinephrine. These studies suggest that luminal dopamine may play a role to modulate sodium transport in the presence of renal nerve activity.