Quantitative and qualitative gender-related differences in jejunal glutathione S-transferase in the rat - Effect of testosterone administration

被引:13
作者
Catania, VA [1 ]
Luquita, MG [1 ]
Pozzi, EJS [1 ]
Mottino, AD [1 ]
机构
[1] Univ Nacl Rosario, Fac Ciencias Bioquim & Farmaceut, CONICET, Inst Fisiol Expt, RA-2000 Rosario, Argentina
关键词
glutathione S-transferases; gender-differences; jejunum; testosterone;
D O I
10.1016/S0024-3205(00)00948-6
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Gender-related differences and the regulation by testosterone of glutathione S-transferase were studied in rat jejunum. We analyzed enzyme activity and the relative content of GST subunits. Four experimental groups of adult rats were studied: normal males, castrated males, castrated males injected with testosterone and normal females. Glutathione S-transferase activity was assayed using 1-chloro-2,4-dinitrobenzene and 1,2-dichloro-4-nitrobenzene as substrates. Differences in subunit composition among groups were evaluated by western blot analysis. The results demonstrated that 1-chloro-2,4-dinitrobenzene conjugation rate is higher in normal males than in normal females and castrated males. Testosterone administration to castrated males raised the activity up to the level observed in normal males. No significant difference in glutathione S-transferase activity towards 1,2-dichloro-4-nitrobenzene was observed among groups. Western blot analysis revealed that males and females differ in all subunits tested that is, rGSTA2, rGSTM1, rGSTM2 and rGSTP1, and that testosterone regulates the content of rGSTM1, rGSTM2 and rGSTP1. In conclusion, jejunal GST shows a gender-dependent regulation affecting both enzyme activity and subunit composition, and testosterone appears to be one of the factors involved. (C) 2000 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:467 / 474
页数:8
相关论文
共 30 条
[1]   DIETARY CARCINOGENS AND ANTICARCINOGENS - OXYGEN RADICALS AND DEGENERATIVE DISEASES [J].
AMES, BN .
SCIENCE, 1983, 221 (4617) :1256-1264
[2]   Differential induction of glutathione S-transferase subunits by spironolactone in rat liver, jejunum and colon [J].
Catania, VA ;
Luquita, MG ;
Pozzi, EJS ;
Mottino, AD .
LIFE SCIENCES, 1998, 63 (26) :2285-2293
[3]   ABSENCE OF HEPATIC P-NITROPHENOL UDP-GLUCURONOSYLTRANSFERASE INDUCTION BY SPIRONOLACTONE IN MALE-RATS - POSSIBLE INVOLVEMENT OF TESTOSTERONE [J].
CATANIA, VA ;
LUQUITA, MG ;
POZZI, EJS ;
FERRI, AM ;
MOTTINO, AD .
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 1992, 70 (11) :1502-1507
[4]   DIETARY LIPIDS INDUCE PHASE 2 ENZYMES IN RAT SMALL-INTESTINE [J].
DANNENBERG, AJ ;
YANG, EK ;
AHARON, D .
BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1210 (01) :8-12
[5]   AGE-ASSOCIATED ALTERATIONS IN HEPATIC GLUTATHIONE-S-TRANSFERASE ACTIVITIES [J].
FUJITA, S ;
KITAGAWA, H ;
ISHIZAWA, H ;
SUZUKI, T ;
KITANI, K .
BIOCHEMICAL PHARMACOLOGY, 1985, 34 (21) :3891-3894
[6]  
GORNALL AG, 1949, J BIOL CHEM, V177, P751
[7]  
HABIG WH, 1974, J BIOL CHEM, V249, P7130
[8]   DIFFERENTIAL REGULATION OF MALE-RAT LIVER GLUTATHIONE S-TRANSFERASES - EFFECTS OF ORCHIDECTOMY AND HORMONE REPLACEMENT [J].
HALES, BF ;
JAIN, R ;
ROBAIRE, B .
BIOCHEMICAL PHARMACOLOGY, 1982, 31 (14) :2389-2393
[9]   The glutathione S-Transferase supergene family: Regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance [J].
Hayes, JD ;
Pulford, DJ .
CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1995, 30 (06) :445-600
[10]  
HOENSCH H, 1976, GASTROENTEROLOGY, V70, P1063