Native chemical ligation using removable Nα-(1-phenyl-2-mercaptoethyl) auxiliaries

被引:131
作者
Botti, P [1 ]
Carrasco, MR [1 ]
Kent, SBH [1 ]
机构
[1] Gryphon Sci, S San Francisco, CA 94080 USA
关键词
D O I
10.1016/S0040-4039(01)00036-3
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
A new methodology to extend native chemical ligation is presented. This method makes use of a novel 1-phenyl-2-mercaptoethyl auxiliary moiety on the alpha -amino group of a first peptide segment, the auxiliary acts as a 1-amino-2-thiol-containing functional group to effect thioester-mediated, amide-forming ligation with a second thioester-containing peptide. Subsequent facile removal of the auxiliary from the newly formed amide gives products with only native peptide structures. (C) 2001 Published by Elsevier Science Ltd.
引用
收藏
页码:1831 / 1833
页数:3
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