Analysis of cyclooxygenase-2 expression in human breast cancer:: high throughput tissue microarray analysis

被引:71
作者
Wülfing, P
Diallo, R
Müller, C
Wülfing, C
Poremba, C
Heinecke, A
Rody, A
Greb, RR
Böcker, W
Kiesel, L
机构
[1] Univ Munster, Dept Obstet & Gynecol, D-48129 Munster, Germany
[2] Univ Munster, Gerhard Domagk Inst Pathol, D-4400 Munster, Germany
[3] Univ Munster, Dept Urol, D-4400 Munster, Germany
[4] Univ Munster, Inst Med Informat & Biomath, D-4400 Munster, Germany
关键词
tissue microarray; breast cancer; cyclooxygenase-2; Cox-2; immunohistochemistry;
D O I
10.1007/s00432-003-0459-1
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Purpose: The objective of this study was to evaluate breast carcinomas for the expression of cyclooxygenase-2 (Cox-2) using a tissue microarray (TMA) and to determine its clinical and prognostic relevance. Methods: We analyzed Cox-2 expression in 600 samples from 200 breast carcinomas immunohistochemically performing TMA technology and semiquantitative analysis. Results were correlated with various clinicopathological variables and follow-up data. Expression of estrogen receptor, progesterone receptor, Ki-67, and Her-2/neu-oncogene was analyzed and correlated with Cox-2 status. Results: We observed a moderate or strong cytoplasmic staining for Cox-2 in 78 (40.6%) of breast carcinomas. Increased Cox-2 expression corresponded to higher pT stage (P=0.038), amplification of Her-2/neu (P=0.032), lymphovascular invasion (P=0.006), a high MIB-1 labeling index (LI) (P<0.001), and histological grading (P = 0.013). We also observed an inverse relationship between strong Cox-2 expression and estrogen and progesterone receptor content of tumors (P=0.037 and P=0.010). However, we could not demonstrate a significant association between Cox-2 staining and overall survival or disease free survival time. Conclusions: These results suggest that Cox-2 expression is significantly associated with less differentiated and more aggressive breast carcinomas and might therefore be a useful prognostic indicator as well as a target for therapy.
引用
收藏
页码:375 / 382
页数:8
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