Controlling protein orientation at interfaces using histidine tags: An alternative to Ni/NTA

被引：86

作者：

Johnson, DL ^{[1
]}

Martin, LL ^{[1
]}

机构：

[1] Monash Univ, Sch Chem, Clayton, Vic 3800, Australia

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 07期

关键词：

D O I：

10.1021/ja045084g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A strategy for immobilizing histidine-tagged proteins at surfaces has been developed by using a macrocyclic chelator that enhances the stability and specificity of conventional histidine-tag technology and allows application of this technology to long-term studies including direct electrochemistry. Copyright © 2005 American Chemical Society.

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页码：2018 / 2019

页数：2

相关论文

共 18 条

[1]

BARD AJ, 1980, ELECTROCHEMICAL METH

[2] MACROCYCLIC EFFECT ON STABILITY OF COPPER(II) TETRAMINE COMPLEXES [J].

CABBINESS, DK ;

MARGERUM, DW .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1969, 91 (23) :6540-+

[3] Twenty-five years of immobilized metal ion affinity chromatography: past, present and future [J].

Chaga, GS .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2001, 49 (1-3) :313-334

[4] Voltammetric peak broadening for cytochrome c/alkanethiolate monolayer structures: Dispersion of formal potentials [J].

Clark, RA ;

Bowden, EF .

LANGMUIR, 1997, 13 (03) :559-565

[5] Ni-NTA-gold clusters target his-tagged proteins [J].

Hainfeld, JF ;

Liu, WQ ;

Halsey, CMR ;

Freimuth, P ;

Powell, RD .

JOURNAL OF STRUCTURAL BIOLOGY, 1999, 127 (02) :185-198

[6] Protein selectivity with immobilized metal ion tacn sorbents: Chromatographic studies with human serum proteins and several other globular proteins [J].

Jiang, W ;

Graham, B ;

Spiccia, L ;

Hearn, MTW .

ANALYTICAL BIOCHEMISTRY, 1998, 255 (01) :47-58

[7] Probing the stability of the disulfide radical intermediate of thioredoxin using direct electrochemistry [J].

Johnson, DL ;

Polyak, SW ;

Wallace, JC ;

Martin, LL .

LETTERS IN PEPTIDE SCIENCE, 2003, 10 (5-6) :495-500

[8] Electrochemical characterization of purified Rhus vernicifera laccase:: Voltammetric evidence for a sequential four-electron transfer [J].

Johnson, DL ;

Thompson, JL ;

Brinkmann, SM ;

Schuller, KA ;

Martin, LL .

BIOCHEMISTRY, 2003, 42 (34) :10229-10237

[9] The influence of promoter and of electrode material on the cyclic voltammetry of Pisum sativum plastocyanin [J].

Johnson, DL ;

Maxwell, CJ ;

Losic, D ;

Shapter, JG ;

Martin, LL .

BIOELECTROCHEMISTRY, 2002, 58 (02) :137-147

[10]

JOHNSON DL, 2004, UNPUB J MOL ENDO