Clonality assessment in blood of patients with mantle cell lymphoma

被引：9

作者：

Bertoni, F ^{[1
]}

Roggero, E ^{[1
]}

Luscieti, P ^{[1
]}

Pedrinis, E ^{[1
]}

Cavalli, F ^{[1
]}

Zucca, E ^{[1
]}

机构：

[1] Osped San Giovanni, Serv Oncol Cantonale, CH-6500 Bellinzona, Switzerland

来源：

LEUKEMIA & LYMPHOMA | 1999年 / 32卷 / 3-4期

关键词：

mantle cell lymphoma; bd-1/PRAD1; t(11; 14); immunoglobulin gene rearrangements; PCR; CDR3;

D O I：

10.3109/10428199909167400

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The presence of circulating neoplastic cells at diagnosis was assessed in the blood of patients presenting with mantle cell lymphoma (MCL) to determine the feasibility of a diagnostic molecular assay. Blood samples from 16 patients with pathologically reviewed MCL were analysed for the t( 11;14)(q13;q32) translocation by the polymerase chain reaction (PCR): 7 (44%) were found positive. The remaining cases were examined by PCR for the presence of circulating neoplastic B-cells by amplifying the third complementarity region (CDR3) of immunoglobulin heavy chain genes and the immunoglobulin light kappa chain deletion rearrangements. A further 7 (44%) patients showed the presence of clonal lymphoma cells, leaving only 2 (12%) of cases negative for circulating lymphomatous cells. This study suggests that there is a high incidence of lymphoma cells in the blood of patients presenting with MCL. PCR for these clonal cells may be diagnostically useful.

引用

页码：375 / 379

页数：5

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