A highly specific one-step PCR-assay for the rapid discrimination of enteropathogenic Yersinia enterocolitica from pathogenic Yersinia pseudotuberculosis and Yersinia pestis

被引：10

作者：

Arnold, T

Hensel, A

Hagen, R

Aleksic, S

Neubauer, H

Scholz, HC

机构：

[1] Inst Anim Hyg & Publ Vet Hlth, D-04103 Leipzig, Germany

[2] Sanitatsakad Bundeswehr, Inst Mikrobiol, Munich, Germany

[3] Natl Reference Ctr Yersiniosis, Inst Hyg, Hamburg, Germany

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 2001年 / 24卷 / 02期

关键词：

Yersinia; yopT; PCR; diagnostic;

D O I：

10.1078/0723-2020-00040

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Based on differences within the yopT-coding region of Yersinia. enterocolitica, Y. pseudotuberculosis and Y pestis, a rapid and sensitive one-step polymerase chain reaction assay with high specificity for pathogenic Y enterocolitica was developed. By this method pathogenic isolates of Y. enterocolitica can be easily identified and discriminated from other members of this genus. The entire coding sequence of the yopT effector gene of Y. pseudotuberculosis Y36 was determined.

引用

收藏

页码：285 / 289

页数：5

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