Nucleoprotein-RNA Orientation in the Measles Virus Nucleocapsid by Three-Dimensional Electron Microscopy

被引：51

作者：

Desfosses, Ambroise ^{[1
]}

Goret, Gael ^{[2
]}

Estrozi, Leandro Farias ^{[1
]}

Ruigrok, Rob W. H. ^{[1
]}

Gutsche, Irina ^{[1
]}

机构：

[1] UJF EMBL CNRS, UVHCI, UMI 3265, F-38042 Grenoble 9, France

[2] CEA CNRS UJF, Inst Biol Struct Jean Pierre Ebel, UMR 5075, Grenoble, France

来源：

JOURNAL OF VIROLOGY | 2011年 / 85卷 / 03期

关键词：

C-TERMINAL DOMAIN; CRYSTAL-STRUCTURE; COMPLEX; BINDING; RECONSTRUCTION;

D O I：

10.1128/JVI.01459-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Recombinant measles virus nucleoprotein-RNA (N-RNA) helices were analyzed by negative-stain electron microscopy. Three-dimensional reconstructions of trypsin-digested and intact nucleocapsids coupled to the docking of the atomic structure of the respiratory syncytial virus (RSV) N-RNA subunit into the electron microscopy density map support a model that places the RNA at the exterior of the helix and the disordered C-terminal domain toward the helix interior, and they suggest the position of the six nucleotides with respect to the measles N protomer.

引用

页码：1391 / 1395

页数：5

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