Structure characterization of three polysaccharides and a comparative study of their immunomodulatory activities on chicken macrophage

被引：34

作者：

Fan, Wentao ^{[1
]}

Zhang, Shijie ^{[2
]}

Hao, Pan ^{[1
]}

Zheng, Pimiao ^{[1
]}

Liu, Jianzhu ^{[3
]}

Zhao, Xiaona ^{[1
]}

机构：

[1] Shandong Agr Univ, Coll Anim Sci & Vet Med, Tai An 271018, Shandong, Peoples R China

[2] Zhengzhou Ninth Peoples Hosp, Zhengzhou 450053, Peoples R China

[3] Shandong Agr Univ, Res Ctr Anim Dis Control Engn Shandong Prov, Tai An 271018, Shandong, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2016年 / 153卷

关键词：

Polysaccharide; Comparative analysis; Structure characterization; Immunological activity; Chicken macrophage; ATRACTYLODIS-MACROCEPHALAE-KOIDZ; CYTOKINES; ADJUVANTS; SINENSIS; ENHANCE;

D O I：

10.1016/j.carbpol.2016.07.116

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

In this study, we evaluated structure characterization and immunomodulatory activity of polysaccharides from Astragalus aboriginum Richardson (RAPS), Atractylodes macrocephala Koidz (RAMPS) and Rumia seseloides Hoffm (RSPS) in vitro on chicken macrophage. We found that molecular weight of RAPS and RAMPS was 122.4 and 109.4 kDa higher than 64.71 kDa of RSPS. Glucose occupied 83.95% and 66.39% in RAPS and RAMPS, respectively. RSPS mainly contained glucose and galacturonic acid, which accounted for 32.35% and 29.25%, respectively. The NMR results displayed that RAPS and RAMPS contained beta- glucose, beta-galactose, and beta-galacturonic acid. The backbone was 1 6 linked glucose. RSPS showed at least six monosaccharide response signals. In vitro experiment, the results showed that RAPS at dosage of 15.62 mu g mL(-1) exhibited significant immunological on chicken macrophage compared to RAMPS and RSPS. Interestingly, costimulatory molecules levels in RSPS group were higher than that of RAPS, which may associated with the special structure of RSPS. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：631 / 640

页数：10

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