Characterisation of glucuronidation and transport in V79 cells co-expressing UGT1A1 and MRP1

被引:10
作者
Cuff, RL
Wade, LT
Rychlik, B
Jedlitschky, GA
Burchell, B [1 ]
机构
[1] Univ Dundee, Ninewells Hosp & Med Sch, Dept Mol & Cellular Pharmacol, Dundee DD1 9SY, Scotland
[2] Univ Lodz, Dept Mol Biophys, PL-90131 Lodz, Poland
[3] Deutsch Krebsforschungszentrum, D-6900 Heidelberg, Germany
关键词
UGT1A1; MRP1; bilirubin glucuronide; estradiol glucuronide; UDP-glucuronosyltransferases;
D O I
10.1016/S0378-4274(01)00305-8
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
The co-ordinated glucuronidation and export of compounds from cells is an important determinant in the detoxification of many compounds in vivo. Many UDP-glucuronosyltransferases (UGTs) and several multidrug resistance proteins (MRPs) have been cloned and individually expressed to assess specificity of glucuronidation and transport. However, to further understand the interplay between glucuronidation and transport we are developing stable cell lines that express different combinations of UGT and MRP isoforms. V79 cells expressing both UGT1A1 and MRP1 have been established. The ability of these cell lines to both glucuronidate and transport compounds was assessed ex vivo using estradiol and bilirubin as substrates. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:43 / 49
页数:7
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