Comparison of two commercial systems (Vitek and MicroScan-WalkAway) for antimicrobial susceptibility testing of Pseudomonas aeruginosa isolates from cystic fibrosis patients

被引:54
作者
Burns, JL
Saiman, L
Whittier, S
Krzewinski, J
Liu, ZL
Larone, D
Marshall, SA
Jones, RN
机构
[1] Univ Washington, Childrens Hosp & Reg Med Ctr, Dept Pediat, Div Infect Dis, Seattle, WA 98105 USA
[2] Columbia Univ, Dept Pediat, Div Infect Dis, New York, NY 10027 USA
[3] New York Presbyterian Hosp, Columbia Presbyterian Ctr, Dept Pathol, New York, NY USA
[4] Univ Iowa, Coll Med, Dept Pathol, Iowa City, IA 52242 USA
关键词
D O I
10.1016/S0732-8893(01)00234-6
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Antimicrobial susceptibility testing of cystic fibrosis (CF) isolates of Pseudomonas aeruginosa is difficult because the organisms are often mucoid and slow-growing. This study of 498 CF strains examined the correlation of results derived from two commonly used commercial systems (Vitek, MicroScan-WalkAway) with a reference method for 10 antimicrobials. Correlation to reference results was unacceptably low for all agents and both commercial systems had a high rate of very major (false-susceptible) errors. Although mucoid strains produced a 4.8% greater intermethod error, it was not markedly different than non-mucoid strains for the Vitek System. Overall, these tested commercial systems performed poorly for CF isolates in contrast to earlier reported, high correlations with the reference methods (broth microdilution frozen panels and agar dilution) of the National Committee fur Clinical Laboratory Standards, the standardized disk diffusion test, and the Etest (AB BIODISK, Solna, Sweden). (C) 2001 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:257 / 260
页数:4
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