Self-recognition of N-linked glycans with multivalent GlcNAc, determined as ceramide mimetic conjugate

被引：11

作者：

Yoon, Seon-Joo

Ikeda, Shoko

Sadilek, Martin

Hakomori, Sen-Itiroh

Ishida, Hideharu

Kiso, Makoto

机构：

[1] NW Res Inst, Seattle, WA 98122 USA

[2] Univ Washington, Pacific NW Res Inst, Div Biomembrane Res, Dept Pathol, Seattle, WA 98195 USA

[3] Gifu Univ, Dept Appl Bio Organ Chem, Gifu 501, Japan

来源：

GLYCOBIOLOGY | 2007年 / 17卷 / 09期

关键词：

aminoceramide; carbohydrate-to-carbohydrate interaction; glycosphingolipid; homotypic interaction; oligosaccharide;

D O I：

10.1093/glycob/cwm069

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Aminoceramide mimetic was synthesized and conjugated to N-linked oligosaccharides having multivalent GlcNAc by reductive amination. Ceramide mimetic conjugates with "complex-type" glycan having five or six GlcNAc termini ( termed Os Fr. B-Cer) were purified, analyzed by thin-layer chromatography (TLC), and finally characterized by MS/MS analysis through liquid chromatography/mass spectrometry. Binding of Os Fr. B-Cer placed on solid phase polystyrene surface with [H-3] cholesterol-labeled liposomes containing Os Fr. B-Cer, or containing various glycosphingolipids (GSLs) was determined. The binding of Os Fr. B-Cer liposomes to Os Fr. B-Cer coated plate was significantly higher than binding of GM3 liposomes. Other GSL liposomes showed no binding. Thus, self-recognition of Os Fr. B-Cer was clearly demonstrated using ceramidemimetic conjugates.

引用

页码：1007 / 1014

页数：8

共 53 条

[1] Germ cell survival through carbohydrate-mediated interaction with Sertoli cells [J].