Extraction and genotyping of Cryptosporidium parvum DNA from fecal smears on glass slides stained conventionally for direct microscope examination

被引：24

作者：

Amar, C

Pedraza-Díaz, S

McLauchlin, J

机构：

[1] Cent Publ Hlth Lab, PHLS Div Gastrointestinal Infect, Food Safety Microbiol Lab, London NW9 5HT, England

[2] Univ Greenwich, Sch Chem & Life Sci, London SE18 8PF, England

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 01期

关键词：

D O I：

10.1128/JCM.39.1.401-403.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A method was developed for extracting cryptosporidial DNA from stained fecal smears on glass microscope slides. The correct genotype of Cryptosporidium parvum was amplified by PCR from 89 (85%) of 105 smears following conventional staining but not from negative controls. This technique may have applications for analysis of other infections agents.

引用

页码：401 / 403

页数：3

共 10 条

[1]

Arrowood M., 1997, CRYPTOSPORIDIUM CRYP, P43

[2] RAPID AND SIMPLE METHOD FOR PURIFICATION OF NUCLEIC-ACIDS [J].