IL-27 alleviates airway remodeling in a mouse model of asthma via PI3K/Akt pathway

被引:36
作者
Li, Xin [1 ]
Zhou, Ling [2 ]
Zhang, Zewen [3 ]
Liu, Yuanyuan [4 ]
Liu, Ju [5 ]
Zhang, Caiqing [2 ]
机构
[1] Weifang Med Univ, Dept Clin Med, Weifang, Shandong, Peoples R China
[2] Shandong Univ, Shandong Prov Qianfoshan Hosp, Dept Resp Med, Jinan, Shandong, Peoples R China
[3] Shandong Univ, MR Shandong Med Imaging Res Inst, Dept MR, Jinan, Shandong, Peoples R China
[4] Shandong First Med Univ, Affiliated Hosp 2, Dept Resp Med, Tai An, Shandong, Peoples R China
[5] Shandong Univ, Shandong Prov Qianfoshan Hosp, Med Res Ctr, Jinan, Shandong, Peoples R China
关键词
Asthma; IL-27; PI3K; Akt; signaling pathway; airway remodeling; PHOSPHOINOSITIDE; 3-KINASE; MECHANISMS; PROLIFERATION; INFLAMMATION; CELLS; THERAPY; STAT1;
D O I
10.1080/01902148.2020.1740356
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
100201 [内科学];
摘要
Purpose: Airway remodeling is one of the features of severe asthma. Previous study shows that IL-27 inhibits airway inflammation in asthmatic mice. However, the role of IL-27 on airway remodeling in OVA-induced asthmatic mice and its possible mechanism remain unclear. Methods: We established an ovalbumin (OVA)-induced asthmatic mice model. IL-27 were preventative administered to OVA-induced asthmatic mice. The total cells in Bronchoalveolar lavage fluid (BALF) and Airway hyperresponsiveness (AHR) were measured. The lung tissues were performed by Hematoxylin and eosin (HE) staining to estimate the pathological changes. Masson staining was used to observe the collagen deposition area. The expression of alpha-smooth muscle actin (alpha-SMA) and Type I collagen was measured by immunohistochemistry, western blot, and quantitative reverse transcription polymerase chain reaction (qRT-PCR). Additionally, western blot was also used to measure the expression of phosphorylated-Akt (p-Akt) in each group. Results: IL-27 group showed significant inhibitory effect on the alpha-SMA and Type I collagen. The expression of p-Akt in the tissues of asthma model was increased and inhibited by IL-27. Conclusions: IL-27 can alleviate airway remodeling in OVA-induced asthmatic mice, and the mechanism may relate to PI3K/Akt pathway.
引用
收藏
页码:98 / 108
页数:11
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