Principal component global analysis of fluorescence and absorption spectra of 2-(2′-hydroxyphenyl)benzimidazole

被引：27

作者：

Al-Soufi, W ^{[1
]}

Novo, M

Mosquera, M

机构：

[1] Univ Santiago de Compostela, Fac Ciencias, Dept Quim Fis, E-27002 Lugo, Spain

[2] Univ Santiago de Compostela, Fac Quim, Dept Quim Fis, E-15706 Santiago, Spain

来源：

APPLIED SPECTROSCOPY | 2001年 / 55卷 / 05期

关键词：

spectroscopic techniques; fluorescence; absorption; acid-base equilibria; 2-(2 '-hydroxyphenyl)benzimidazole; computer application; global analysis; principal component analysis;

D O I：

10.1366/0003702011952253

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Principal component analysis (PCA) of a series of absorption and emission spectra of 2-(2'-hydroxyphenyl)benzimidazole (HBI) in aqueous solutions in the PH range from 1.11 to 12.3 and 3.85 to 9.62, respectively, reveals the presence of three contributing components both in the ground and in the excited state. The combination of PCA with global analysis (PCGA) confirms the previously proposed reaction mechanism and allows the determination of pK(a) values, pure component spectra, and concentration profiles of the ground- and excited-state species in spite of their strong spectral overlap. PCGA is proved to be a powerful complement to standard nonlinear least-squares analysis of physicochemical systems.

引用

页码：630 / 636

页数：7

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