Evaluation of antibody responses of emus (Dromaius novaehollandiae) to avian influenza virus infection

被引：8

作者：

Zhou, EM ^{[1
]}

Chan, M ^{[1
]}

McIsaac, M ^{[1
]}

Heckert, RA ^{[1
]}

机构：

[1] Canadian Food Inspect Agcy, Anim Dis Res Inst, Virol Sect, Nepear, ON, Canada

来源：

AVIAN DISEASES | 1998年 / 42卷 / 04期

关键词：

anti-NP antibodies; avian influenza virus; C-ELISA; ELISA; emu; immunoassay; nucleoprotein; ratites; serodiagnosis;

D O I：

10.2307/1592712

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Emu antibody responses to avian influenza virus (AIV) infection were evaluated by the competitive enzyme-linked immunosorbent assay (C-ELISA), agar gel immunodiffusion (AGID) and hemagglutination inhibition (HI) tests. All birds infected with AIV H5N1, H5N3, or H7N7 developed antinucleoprotein (NP) antibodies as early as 7 days postinfection as detected by the C-ELISA. The responses lasted 49 days for the emus receiving H5N3 and at least 56 days for emus receiving the other two viruses. By evaluating 50 emu field serum samples, the C-ELISA was found more sensitive than the ACID test for the detection of anti-NP antibodies. This study indicates that emus experimentally infected with AIV developed antibody responses that can be detected by C-ELISA, ACID, and HI tests. The results from this and our previous studies demonstrate the use of the C-ELISA as a substitute for the ACID test in a routine serodiagnostic screening for detection of antibodies to AIV infection in multiple avian species.

引用

页码：757 / 761

页数：5

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