Oligonucleotide-stabilized Ag nanocluster fluorophores

被引：795

作者：

Richards, Chris I. ^{[1
,2
]}

Choi, Sungmoon ^{[1
,2
]}

Hsiang, Jung-Cheng ^{[1
,2
]}

Antoku, Yasuko ^{[1
,2
]}

Vosch, Tom ^{[1
,2
]}

Bongiorno, Angelo ^{[1
,2
]}

Tzeng, Yih-Ling ^{[3
]}

Dickson, Robert M. ^{[1
,2
]}

机构：

[1] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA

[2] Georgia Inst Technol, Petit Inst Biosci & Bioengn, Atlanta, GA 30332 USA

[3] Emory Univ, Dept Med, Atlanta, GA 30322 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 15期

关键词：

D O I：

10.1021/ja8005644

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Single-stranded oligonucleotides stabilize highly fluorescent Ag nanoclusters, with emission colors tunable via DNA sequence. We utilized DNA microarrays to optimize these scaffold sequences for creating nearly spectrally pure Ag nanocluster fluorophores that are highly photostable and exhibit great buffer stability. Five different nanocluster emitters have been created with tunable emission from the blue to the near-IR and excellent photophysical properties. Ensemble and single molecule fluorescence studies show that oligonucleotide encapsulated Ag nanoclusters exhibit significantly greater photostability and higher emission rates than commonly used cyanine dyes.

引用

页码：5038 / +

页数：3

共 22 条

[1] [Anonymous], THESIS GEORGIA I TEC
[2] Semiconductor nanocrystals as fluorescent biological labels
Bruchez, M
Moronne, M
Gin, P
Weiss, S
Alivisatos, AP
[J]. SCIENCE, 1998, 281 (5385) : 2013 - 2016
[3] Quantum dot bioconjugates for ultrasensitive nonisotopic detection
Chan, WCW
Nie, SM
[J]. SCIENCE, 1998, 281 (5385) : 2016 - 2018
[4] Derfus AM, 2004, NANO LETT, V4, P11, DOI 10.1021/nl0347334
[5] Photobleaching of fluorescent dyes under conditions used for single-molecule detection: Evidence of two-step photolysis
Eggeling, C
Widengren, J
Rigler, R
Seidel, CAM
[J]. ANALYTICAL CHEMISTRY, 1998, 70 (13) : 2651 - 2659
[6] Probing transcription factor dynamics at the single-molecule level in a living cell
Elf, Johan
Li, Gene-Wei
Xie, X. Sunney
[J]. SCIENCE, 2007, 316 (5828) : 1191 - 1194
[7] Illuminating the mechanistic roles of enzyme conformational dynamics
Hanson, Jeffrey A.
Duderstadt, Karl
Watkins, Lucas P.
Bhattacharyya, Sucharita
Brokaw, Jason
Chu, Jhih-Wei
Yang, Haw
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (46) : 18055 - 18060
[8] Nonexponential "blinking" kinetics of single CdSe quantum dots: A universal power law behavior
Kuno, M
Fromm, DP
Hamann, HF
Gallagher, A
Nesbitt, DJ
[J]. JOURNAL OF CHEMICAL PHYSICS, 2000, 112 (07) : 3117 - 3120
[9] Photodynamics of a single quantum dot: Fluorescence activation, enhancement, intermittency, and decay
Lee, Steven F.
Osborne, Mark A.
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (29) : 8936 - +
[10] Single-molecule measurement of protein folding kinetics
Lipman, EA
Schuler, B
Bakajin, O
Eaton, WA
[J]. SCIENCE, 2003, 301 (5637) : 1233 - 1235

← 1 2 3 →