Preparation and characterization of monolithic polymer columns for capillary electrochromatography

被引：103

作者：

Jiang, T ^{[1
]}

Jiskra, J ^{[1
]}

Claessens, HA ^{[1
]}

Cramers, CA ^{[1
]}

机构：

[1] Eindhoven Univ Technol, Dept Chem & Chem Engn, Instrumental Anal Lab, NL-5600 MB Eindhoven, Netherlands

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 923卷 / 1-2期

关键词：

electrochromatography; monolithic polymer column; porosity; micropore;

D O I：

10.1016/S0021-9673(01)00982-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of micro-monolithic columns with different porosities were prepared for capillary electrochromatography (CEC) by in-situ copolymerization of butyl methacrylate, ethylene glycol dimethacrylate, and 2-acrylamido-2-methyl-1-propanesulfonic acid in the presence of a porogen in fused-silica capillaries of 100 mum I.D. Different column porosities were obtained by changing the ratios of monomers to porogenic solvents. Columns were investigated and evaluated under both pressure-driven (high-performance liquid chromatography, HPLC) and electro-driven (capillary electrochromatography, CEC) conditions. Each column exhibited different efficiency and dependency on flow velocity under electro-driven conditions. Abnormally broad peaks for some relatively bulky molecules were observed. Possible explanations are discussed. The differences in column efficiency and retention behavior between the two eluent-driven modes were studied in detail. In addition, other column proper-ties, such as morphology, porosity, stability and reproducibility, were extensively tested. (C) 2001 Elsevier Science B.V. rights reserved.

引用

页码：215 / 227

页数：13

共 57 条

[1] A review of the current status of capillary electrochromatography technology and applications [J].