C3 molecules internalize and enhance the growth of Lewis lung carcinoma cells

被引:7
作者
di Renzo, L
Longo, A
Morgante, E
Mardente, S
Prodinger, WM
Russo, M
Pontieri, GM
Lipari, M
机构
[1] Univ Roma La Sapienza, Dept Expt Med & Pathol, Rome, Italy
[2] Univ Innsbruck, Inst Hyg, A-6020 Innsbruck, Austria
关键词
D O I
10.1016/S0171-2985(99)80035-7
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
C3 molecules from normal murine serum are mainly bound to Lewis lung carcinoma cells (3LL) that do not express CRs, mainly through covalent binding as determined by the appearance of bands stained with anti-C3 and larger than 190 kD in immunoblots of proteins in whole cell extracts. Methylamine-treated, or zymosan-treated normal mouse serum, heat inactivated, or EDTA-treated murine serum resulted in low C3 deposition on 3LL cells, as indicated by fluorescence tests and immunoblotting. Cytofluorimetric studies showed that C3 molecules bound to 3LL cells were internalized in a time- and temperature-dependent process. This was confirmed by electronmicroscopic studies. The conditions allowing C3 fixation to acceptor sites and subsequent internalization increased cell proliferation. This was also true, when serum from mice genetically deficient in C5 was used which stresses the role of C3 in contrast to effects of membrane attack complex formation.
引用
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页码:92 / 105
页数:14
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